Pre-storage and Release of Purification Reagents for Full “hands-off” Integration of Dna/rna Assays on the Labdisk Platform
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چکیده
For the first time we demonstrate complete integration and pre-storage of all required components for nucleic acid extraction and purification on the centrifugal microfluidic LabDisk platform. On the one hand pre-storage of large volume liquid reagents from 200-–-550 μL is realized in stickpacks, that are stacked to decrease the footprint consumption on the LabDisk and are released automatically during the run using centrifugal pressure (pcent-=-1 bar). On the other hand magnetic beads are mixed with PEG8000 (ratio 2:1) and air dried into the final cavity over 12 h. PEG8000 allows stable pre-storage and does not influence the extraction yield.
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تاریخ انتشار 2014